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cell lines u 2 os american type culture collection htb 96 jurkat  (ATCC)


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    ATCC cell lines u 2 os american type culture collection htb 96 jurkat
    Cell Lines U 2 Os American Type Culture Collection Htb 96 Jurkat, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 8509 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+lines+u+2+os+american+type+culture+collection+htb+96+jurkat/U-2+OS/pm41926287-1021-149-153
    Average 99 stars, based on 8509 article reviews
    cell lines u 2 os american type culture collection htb 96 jurkat - by Bioz Stars, 2026-09
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    Flow Cytometry:

    Article Title: Unveiling the molecular architecture of T cells and immune synapses with cryo-expansion microscopy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies α-tubulin, rabbit ABCD antibodies Cat# AA345, clone scFv-F2C; RRID:AB_3106979 β-tubulin, mouse ABCD antibodies Cat# AA344, clone scFv-S11B; RRID:AB_3678982 β-actin, mouse Proteintech Cat# 60008-1-1, different lots; RRID:AB_2289225 30α-tubulin, rabbit Abcam Cat# ab18251, different lots; RRID:AB_2210057 TOMM20, rabbit Abcam Cat# ab186734, different lots; RRID:AB_2716623 LAMP1, sheep R and D systems Cat# AF4800, different lots; RRID:AB_1026176 C2CD3, rabbit Atlas antibodies Cat# HPA038552, different lots; RRID:AB_10669542 CEP164, rabbit Proteintech Cat# 22227-1-AP; RRID:AB_2651175 CEP170, rabbit Proteintech Cat# 27325-1-AP; RRID:AB_2880844 Granzyme B, goat R and D systems Cat# AF2906-SP; RRID:AB_622003 Granzyme B, mouse R and D systems Cat# MAB2906-SP, clone 351927; RRID:AB_2263752 Perforin, rabbit Proteintech Cat# 14580-1-AP, multiple lots; RRID:AB_10639524 CD8, mouse Proteintech Cat# 66868-1-Ig, clone 1G2B10; RRID:AB_2882205 CD4, rabbit Abcam Cat# ab133616; RRID:AB_2750883 CD2, rabbit Proteintech Cat# 23973-1-AP; RRID:AB_2879384 LCK, rabbit Atlas antibody Cat# HPA003494; RRID:AB_1852751 RAB7A, rabbit Proteintech Cat# 55469-1-AP; RRID:AB_11182831 RAB27A, rabbit Proteintech Cat# 66058-1-Ig; RRID:AB_11042596 VAMP7, rabbit Proteintech Cat# 22268-1-AP; RRID:AB_11182718 STX11, rabbit Proteintech Cat# 13301-1-AP; RRID:AB_2198214 SYTL2, rabbit Proteintech Cat# 12359-1-AP; RRID:AB_11182492 UNC13D, rabbit Proteintech Cat# 16905-1-AP; RRID:AB_2212713 TSP1, rabbit monoclonal Cell Signaling Cat# 37879, clone D7E5F; RRID:AB_2799123 Anti-mouse Alexa Fluor 568, goat Invitrogen Cat# A11029; RRID:AB_2534088 Anti-mouse Alexa Fluor 488, goat Invitrogen Cat# A11001; RRID:AB_2534069 Anti-Rabbit Alexa Fluor 488, goat Invitrogen Cat# A11008; RRID:AB_143165 Anti-Rabbit Alexa Fluor 568, goat Invitrogen Cat# A11036; RRID:AB_10563566 Anti-sheep Alexa Fluor 488, donkey Invitrogen Cat# A11015; RRID:AB_141362 Anti-rabbit Alexa Fluor Cy3, donkey Jackson Cat# 711-165-152; RRID:AB_2307443 Anti-Mouse Alexa Fluor 647, donkey Jackson Cat# 715-605-151; RRID:AB_2340863 Anti-Rabbit Alexa Fluor 647, donkey ThermoFisher Cat# A31573; RRID:AB_2536183 anti CD3 Alexa Fluor 700, flow cytometry BioLegend Cat# 300324; RRID:AB_493739 anti-CD4 − Pe-Cy7, flow cytometry BioLegend Cat# 317414; RRID:AB_571959 CD8, BV605, flow cytometry BioLegend Cat# 301040; RRID:AB_2563185 (Continued on next page) 22 Cell Reports 45, 117165, April 28, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER mouse TCRbeta- PE, flow cytometry BioLegend Cat#, 109208; RRID:AB_313431 Granzyme B- FITC, flow cytometry BD Cat#, 560211; RRID:AB_1645488 Perforin Alexa 647, flow cytometry BD Cat#, 563576; RRID:AB_2738287 Biological samples periphereal blood mononuclear cells from buffy coates of healthy human donors, completely anonymized and in Center of Interregional Blood Transfusion SRK Bern protocol P_503 human glioblastoma samples and melanoma brain metastass samples Geneva University Hospital protocol 2022-02109 protocol 2025-00256 Chemicals, peptides, and recombinant proteins Sodium acrylate home made no identifier available ICAM-1 Dustin lab, kind gift no identifier available mCLING-ATTO-Alexa Fluor 647N Source: Synaptic Systems Cat# 710 006AT1 NHS-Ester-Alexa Fluor 647 Source: Thermo Fisher Scientific Cat# A20006 NHS-Ester-Alexa Fluor 405 Source: Thermo Fisher Scientific Cat# A30000 Critical commercial assays CD4 isolation kit Miltenyi Biotech Cat# 130-096-533 CD8 isolation kit Miltenyi Biotech Cat# 130-096-495 Deposited data Jurlat, CTL SEM datasets OpenOrganelle jrc_jurkat-1, jrc_ctl-id8-1, jrc_ctl-id8-2 Experimental models: Cell lines U-2 OS American Type Culture Collection HTB-96 Jurkat, Clone E6-1 TIB-152 LGC STANDARDS GMBH TIB-152 B cell precursor leukemia BV173 German Cell Culture Collection (DSMZ) ACC 20 Recombinant DNA PEG plasmid Arber lab 101 available upon request PAM plasmid Arber lab 101 available upon request TCR plasmid Arber lab 101 available upon request TCR8 plasmid Arber lab 90 available upon request Software and algorithms Graph Pad Prism GraphPad Software, Inc. RRID:SCR_002798 Fiji NIH RRID:SCR_002285 R statistical environment R Foundation for Statistical Computing RRID:SCR_001905 LAS X software Leica Microsystems RRID:SCR_013673 Leica Thunder Computational Clearing (LVCC) Leica Microsystems Proprietary Imaris 10.1 Bitplane (Oxford Instruments) RRID:SCR_007370 LABKIT plugin (Fiji) Fiji/University of Heidelberg https://imagej.net/plugins/labkit 3D Object Manager (Fiji) ImageJ https://imagej.nih.gov/ij/plugins/track/ objects.html 3D MultiColoc (Fiji) ImageJ https://imagej.nih.gov/ij/plugins/multicolor- colocalization.html CropAndResize plugin CentrioleLab GitHub: https://github.com/CentrioleLab PickCentrioleDim plugin CentrioleLab GitHub: U-ExM-synapse-segmentation macro Cellular Immuno-Oncology Lausanne Zenodo: https://doi.org/10.5281/zenodo. ..

    Recombinant:

    Article Title: Unveiling the molecular architecture of T cells and immune synapses with cryo-expansion microscopy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies α-tubulin, rabbit ABCD antibodies Cat# AA345, clone scFv-F2C; RRID:AB_3106979 β-tubulin, mouse ABCD antibodies Cat# AA344, clone scFv-S11B; RRID:AB_3678982 β-actin, mouse Proteintech Cat# 60008-1-1, different lots; RRID:AB_2289225 30α-tubulin, rabbit Abcam Cat# ab18251, different lots; RRID:AB_2210057 TOMM20, rabbit Abcam Cat# ab186734, different lots; RRID:AB_2716623 LAMP1, sheep R and D systems Cat# AF4800, different lots; RRID:AB_1026176 C2CD3, rabbit Atlas antibodies Cat# HPA038552, different lots; RRID:AB_10669542 CEP164, rabbit Proteintech Cat# 22227-1-AP; RRID:AB_2651175 CEP170, rabbit Proteintech Cat# 27325-1-AP; RRID:AB_2880844 Granzyme B, goat R and D systems Cat# AF2906-SP; RRID:AB_622003 Granzyme B, mouse R and D systems Cat# MAB2906-SP, clone 351927; RRID:AB_2263752 Perforin, rabbit Proteintech Cat# 14580-1-AP, multiple lots; RRID:AB_10639524 CD8, mouse Proteintech Cat# 66868-1-Ig, clone 1G2B10; RRID:AB_2882205 CD4, rabbit Abcam Cat# ab133616; RRID:AB_2750883 CD2, rabbit Proteintech Cat# 23973-1-AP; RRID:AB_2879384 LCK, rabbit Atlas antibody Cat# HPA003494; RRID:AB_1852751 RAB7A, rabbit Proteintech Cat# 55469-1-AP; RRID:AB_11182831 RAB27A, rabbit Proteintech Cat# 66058-1-Ig; RRID:AB_11042596 VAMP7, rabbit Proteintech Cat# 22268-1-AP; RRID:AB_11182718 STX11, rabbit Proteintech Cat# 13301-1-AP; RRID:AB_2198214 SYTL2, rabbit Proteintech Cat# 12359-1-AP; RRID:AB_11182492 UNC13D, rabbit Proteintech Cat# 16905-1-AP; RRID:AB_2212713 TSP1, rabbit monoclonal Cell Signaling Cat# 37879, clone D7E5F; RRID:AB_2799123 Anti-mouse Alexa Fluor 568, goat Invitrogen Cat# A11029; RRID:AB_2534088 Anti-mouse Alexa Fluor 488, goat Invitrogen Cat# A11001; RRID:AB_2534069 Anti-Rabbit Alexa Fluor 488, goat Invitrogen Cat# A11008; RRID:AB_143165 Anti-Rabbit Alexa Fluor 568, goat Invitrogen Cat# A11036; RRID:AB_10563566 Anti-sheep Alexa Fluor 488, donkey Invitrogen Cat# A11015; RRID:AB_141362 Anti-rabbit Alexa Fluor Cy3, donkey Jackson Cat# 711-165-152; RRID:AB_2307443 Anti-Mouse Alexa Fluor 647, donkey Jackson Cat# 715-605-151; RRID:AB_2340863 Anti-Rabbit Alexa Fluor 647, donkey ThermoFisher Cat# A31573; RRID:AB_2536183 anti CD3 Alexa Fluor 700, flow cytometry BioLegend Cat# 300324; RRID:AB_493739 anti-CD4 − Pe-Cy7, flow cytometry BioLegend Cat# 317414; RRID:AB_571959 CD8, BV605, flow cytometry BioLegend Cat# 301040; RRID:AB_2563185 (Continued on next page) 22 Cell Reports 45, 117165, April 28, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER mouse TCRbeta- PE, flow cytometry BioLegend Cat#, 109208; RRID:AB_313431 Granzyme B- FITC, flow cytometry BD Cat#, 560211; RRID:AB_1645488 Perforin Alexa 647, flow cytometry BD Cat#, 563576; RRID:AB_2738287 Biological samples periphereal blood mononuclear cells from buffy coates of healthy human donors, completely anonymized and in Center of Interregional Blood Transfusion SRK Bern protocol P_503 human glioblastoma samples and melanoma brain metastass samples Geneva University Hospital protocol 2022-02109 protocol 2025-00256 Chemicals, peptides, and recombinant proteins Sodium acrylate home made no identifier available ICAM-1 Dustin lab, kind gift no identifier available mCLING-ATTO-Alexa Fluor 647N Source: Synaptic Systems Cat# 710 006AT1 NHS-Ester-Alexa Fluor 647 Source: Thermo Fisher Scientific Cat# A20006 NHS-Ester-Alexa Fluor 405 Source: Thermo Fisher Scientific Cat# A30000 Critical commercial assays CD4 isolation kit Miltenyi Biotech Cat# 130-096-533 CD8 isolation kit Miltenyi Biotech Cat# 130-096-495 Deposited data Jurlat, CTL SEM datasets OpenOrganelle jrc_jurkat-1, jrc_ctl-id8-1, jrc_ctl-id8-2 Experimental models: Cell lines U-2 OS American Type Culture Collection HTB-96 Jurkat, Clone E6-1 TIB-152 LGC STANDARDS GMBH TIB-152 B cell precursor leukemia BV173 German Cell Culture Collection (DSMZ) ACC 20 Recombinant DNA PEG plasmid Arber lab 101 available upon request PAM plasmid Arber lab 101 available upon request TCR plasmid Arber lab 101 available upon request TCR8 plasmid Arber lab 90 available upon request Software and algorithms Graph Pad Prism GraphPad Software, Inc. RRID:SCR_002798 Fiji NIH RRID:SCR_002285 R statistical environment R Foundation for Statistical Computing RRID:SCR_001905 LAS X software Leica Microsystems RRID:SCR_013673 Leica Thunder Computational Clearing (LVCC) Leica Microsystems Proprietary Imaris 10.1 Bitplane (Oxford Instruments) RRID:SCR_007370 LABKIT plugin (Fiji) Fiji/University of Heidelberg https://imagej.net/plugins/labkit 3D Object Manager (Fiji) ImageJ https://imagej.nih.gov/ij/plugins/track/ objects.html 3D MultiColoc (Fiji) ImageJ https://imagej.nih.gov/ij/plugins/multicolor- colocalization.html CropAndResize plugin CentrioleLab GitHub: https://github.com/CentrioleLab PickCentrioleDim plugin CentrioleLab GitHub: U-ExM-synapse-segmentation macro Cellular Immuno-Oncology Lausanne Zenodo: https://doi.org/10.5281/zenodo. ..

    Isolation:

    Article Title: Unveiling the molecular architecture of T cells and immune synapses with cryo-expansion microscopy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies α-tubulin, rabbit ABCD antibodies Cat# AA345, clone scFv-F2C; RRID:AB_3106979 β-tubulin, mouse ABCD antibodies Cat# AA344, clone scFv-S11B; RRID:AB_3678982 β-actin, mouse Proteintech Cat# 60008-1-1, different lots; RRID:AB_2289225 30α-tubulin, rabbit Abcam Cat# ab18251, different lots; RRID:AB_2210057 TOMM20, rabbit Abcam Cat# ab186734, different lots; RRID:AB_2716623 LAMP1, sheep R and D systems Cat# AF4800, different lots; RRID:AB_1026176 C2CD3, rabbit Atlas antibodies Cat# HPA038552, different lots; RRID:AB_10669542 CEP164, rabbit Proteintech Cat# 22227-1-AP; RRID:AB_2651175 CEP170, rabbit Proteintech Cat# 27325-1-AP; RRID:AB_2880844 Granzyme B, goat R and D systems Cat# AF2906-SP; RRID:AB_622003 Granzyme B, mouse R and D systems Cat# MAB2906-SP, clone 351927; RRID:AB_2263752 Perforin, rabbit Proteintech Cat# 14580-1-AP, multiple lots; RRID:AB_10639524 CD8, mouse Proteintech Cat# 66868-1-Ig, clone 1G2B10; RRID:AB_2882205 CD4, rabbit Abcam Cat# ab133616; RRID:AB_2750883 CD2, rabbit Proteintech Cat# 23973-1-AP; RRID:AB_2879384 LCK, rabbit Atlas antibody Cat# HPA003494; RRID:AB_1852751 RAB7A, rabbit Proteintech Cat# 55469-1-AP; RRID:AB_11182831 RAB27A, rabbit Proteintech Cat# 66058-1-Ig; RRID:AB_11042596 VAMP7, rabbit Proteintech Cat# 22268-1-AP; RRID:AB_11182718 STX11, rabbit Proteintech Cat# 13301-1-AP; RRID:AB_2198214 SYTL2, rabbit Proteintech Cat# 12359-1-AP; RRID:AB_11182492 UNC13D, rabbit Proteintech Cat# 16905-1-AP; RRID:AB_2212713 TSP1, rabbit monoclonal Cell Signaling Cat# 37879, clone D7E5F; RRID:AB_2799123 Anti-mouse Alexa Fluor 568, goat Invitrogen Cat# A11029; RRID:AB_2534088 Anti-mouse Alexa Fluor 488, goat Invitrogen Cat# A11001; RRID:AB_2534069 Anti-Rabbit Alexa Fluor 488, goat Invitrogen Cat# A11008; RRID:AB_143165 Anti-Rabbit Alexa Fluor 568, goat Invitrogen Cat# A11036; RRID:AB_10563566 Anti-sheep Alexa Fluor 488, donkey Invitrogen Cat# A11015; RRID:AB_141362 Anti-rabbit Alexa Fluor Cy3, donkey Jackson Cat# 711-165-152; RRID:AB_2307443 Anti-Mouse Alexa Fluor 647, donkey Jackson Cat# 715-605-151; RRID:AB_2340863 Anti-Rabbit Alexa Fluor 647, donkey ThermoFisher Cat# A31573; RRID:AB_2536183 anti CD3 Alexa Fluor 700, flow cytometry BioLegend Cat# 300324; RRID:AB_493739 anti-CD4 − Pe-Cy7, flow cytometry BioLegend Cat# 317414; RRID:AB_571959 CD8, BV605, flow cytometry BioLegend Cat# 301040; RRID:AB_2563185 (Continued on next page) 22 Cell Reports 45, 117165, April 28, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER mouse TCRbeta- PE, flow cytometry BioLegend Cat#, 109208; RRID:AB_313431 Granzyme B- FITC, flow cytometry BD Cat#, 560211; RRID:AB_1645488 Perforin Alexa 647, flow cytometry BD Cat#, 563576; RRID:AB_2738287 Biological samples periphereal blood mononuclear cells from buffy coates of healthy human donors, completely anonymized and in Center of Interregional Blood Transfusion SRK Bern protocol P_503 human glioblastoma samples and melanoma brain metastass samples Geneva University Hospital protocol 2022-02109 protocol 2025-00256 Chemicals, peptides, and recombinant proteins Sodium acrylate home made no identifier available ICAM-1 Dustin lab, kind gift no identifier available mCLING-ATTO-Alexa Fluor 647N Source: Synaptic Systems Cat# 710 006AT1 NHS-Ester-Alexa Fluor 647 Source: Thermo Fisher Scientific Cat# A20006 NHS-Ester-Alexa Fluor 405 Source: Thermo Fisher Scientific Cat# A30000 Critical commercial assays CD4 isolation kit Miltenyi Biotech Cat# 130-096-533 CD8 isolation kit Miltenyi Biotech Cat# 130-096-495 Deposited data Jurlat, CTL SEM datasets OpenOrganelle jrc_jurkat-1, jrc_ctl-id8-1, jrc_ctl-id8-2 Experimental models: Cell lines U-2 OS American Type Culture Collection HTB-96 Jurkat, Clone E6-1 TIB-152 LGC STANDARDS GMBH TIB-152 B cell precursor leukemia BV173 German Cell Culture Collection (DSMZ) ACC 20 Recombinant DNA PEG plasmid Arber lab 101 available upon request PAM plasmid Arber lab 101 available upon request TCR plasmid Arber lab 101 available upon request TCR8 plasmid Arber lab 90 available upon request Software and algorithms Graph Pad Prism GraphPad Software, Inc. RRID:SCR_002798 Fiji NIH RRID:SCR_002285 R statistical environment R Foundation for Statistical Computing RRID:SCR_001905 LAS X software Leica Microsystems RRID:SCR_013673 Leica Thunder Computational Clearing (LVCC) Leica Microsystems Proprietary Imaris 10.1 Bitplane (Oxford Instruments) RRID:SCR_007370 LABKIT plugin (Fiji) Fiji/University of Heidelberg https://imagej.net/plugins/labkit 3D Object Manager (Fiji) ImageJ https://imagej.nih.gov/ij/plugins/track/ objects.html 3D MultiColoc (Fiji) ImageJ https://imagej.nih.gov/ij/plugins/multicolor- colocalization.html CropAndResize plugin CentrioleLab GitHub: https://github.com/CentrioleLab PickCentrioleDim plugin CentrioleLab GitHub: U-ExM-synapse-segmentation macro Cellular Immuno-Oncology Lausanne Zenodo: https://doi.org/10.5281/zenodo. ..

    Cell Culture:

    Article Title: Unveiling the molecular architecture of T cells and immune synapses with cryo-expansion microscopy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies α-tubulin, rabbit ABCD antibodies Cat# AA345, clone scFv-F2C; RRID:AB_3106979 β-tubulin, mouse ABCD antibodies Cat# AA344, clone scFv-S11B; RRID:AB_3678982 β-actin, mouse Proteintech Cat# 60008-1-1, different lots; RRID:AB_2289225 30α-tubulin, rabbit Abcam Cat# ab18251, different lots; RRID:AB_2210057 TOMM20, rabbit Abcam Cat# ab186734, different lots; RRID:AB_2716623 LAMP1, sheep R and D systems Cat# AF4800, different lots; RRID:AB_1026176 C2CD3, rabbit Atlas antibodies Cat# HPA038552, different lots; RRID:AB_10669542 CEP164, rabbit Proteintech Cat# 22227-1-AP; RRID:AB_2651175 CEP170, rabbit Proteintech Cat# 27325-1-AP; RRID:AB_2880844 Granzyme B, goat R and D systems Cat# AF2906-SP; RRID:AB_622003 Granzyme B, mouse R and D systems Cat# MAB2906-SP, clone 351927; RRID:AB_2263752 Perforin, rabbit Proteintech Cat# 14580-1-AP, multiple lots; RRID:AB_10639524 CD8, mouse Proteintech Cat# 66868-1-Ig, clone 1G2B10; RRID:AB_2882205 CD4, rabbit Abcam Cat# ab133616; RRID:AB_2750883 CD2, rabbit Proteintech Cat# 23973-1-AP; RRID:AB_2879384 LCK, rabbit Atlas antibody Cat# HPA003494; RRID:AB_1852751 RAB7A, rabbit Proteintech Cat# 55469-1-AP; RRID:AB_11182831 RAB27A, rabbit Proteintech Cat# 66058-1-Ig; RRID:AB_11042596 VAMP7, rabbit Proteintech Cat# 22268-1-AP; RRID:AB_11182718 STX11, rabbit Proteintech Cat# 13301-1-AP; RRID:AB_2198214 SYTL2, rabbit Proteintech Cat# 12359-1-AP; RRID:AB_11182492 UNC13D, rabbit Proteintech Cat# 16905-1-AP; RRID:AB_2212713 TSP1, rabbit monoclonal Cell Signaling Cat# 37879, clone D7E5F; RRID:AB_2799123 Anti-mouse Alexa Fluor 568, goat Invitrogen Cat# A11029; RRID:AB_2534088 Anti-mouse Alexa Fluor 488, goat Invitrogen Cat# A11001; RRID:AB_2534069 Anti-Rabbit Alexa Fluor 488, goat Invitrogen Cat# A11008; RRID:AB_143165 Anti-Rabbit Alexa Fluor 568, goat Invitrogen Cat# A11036; RRID:AB_10563566 Anti-sheep Alexa Fluor 488, donkey Invitrogen Cat# A11015; RRID:AB_141362 Anti-rabbit Alexa Fluor Cy3, donkey Jackson Cat# 711-165-152; RRID:AB_2307443 Anti-Mouse Alexa Fluor 647, donkey Jackson Cat# 715-605-151; RRID:AB_2340863 Anti-Rabbit Alexa Fluor 647, donkey ThermoFisher Cat# A31573; RRID:AB_2536183 anti CD3 Alexa Fluor 700, flow cytometry BioLegend Cat# 300324; RRID:AB_493739 anti-CD4 − Pe-Cy7, flow cytometry BioLegend Cat# 317414; RRID:AB_571959 CD8, BV605, flow cytometry BioLegend Cat# 301040; RRID:AB_2563185 (Continued on next page) 22 Cell Reports 45, 117165, April 28, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER mouse TCRbeta- PE, flow cytometry BioLegend Cat#, 109208; RRID:AB_313431 Granzyme B- FITC, flow cytometry BD Cat#, 560211; RRID:AB_1645488 Perforin Alexa 647, flow cytometry BD Cat#, 563576; RRID:AB_2738287 Biological samples periphereal blood mononuclear cells from buffy coates of healthy human donors, completely anonymized and in Center of Interregional Blood Transfusion SRK Bern protocol P_503 human glioblastoma samples and melanoma brain metastass samples Geneva University Hospital protocol 2022-02109 protocol 2025-00256 Chemicals, peptides, and recombinant proteins Sodium acrylate home made no identifier available ICAM-1 Dustin lab, kind gift no identifier available mCLING-ATTO-Alexa Fluor 647N Source: Synaptic Systems Cat# 710 006AT1 NHS-Ester-Alexa Fluor 647 Source: Thermo Fisher Scientific Cat# A20006 NHS-Ester-Alexa Fluor 405 Source: Thermo Fisher Scientific Cat# A30000 Critical commercial assays CD4 isolation kit Miltenyi Biotech Cat# 130-096-533 CD8 isolation kit Miltenyi Biotech Cat# 130-096-495 Deposited data Jurlat, CTL SEM datasets OpenOrganelle jrc_jurkat-1, jrc_ctl-id8-1, jrc_ctl-id8-2 Experimental models: Cell lines U-2 OS American Type Culture Collection HTB-96 Jurkat, Clone E6-1 TIB-152 LGC STANDARDS GMBH TIB-152 B cell precursor leukemia BV173 German Cell Culture Collection (DSMZ) ACC 20 Recombinant DNA PEG plasmid Arber lab 101 available upon request PAM plasmid Arber lab 101 available upon request TCR plasmid Arber lab 101 available upon request TCR8 plasmid Arber lab 90 available upon request Software and algorithms Graph Pad Prism GraphPad Software, Inc. RRID:SCR_002798 Fiji NIH RRID:SCR_002285 R statistical environment R Foundation for Statistical Computing RRID:SCR_001905 LAS X software Leica Microsystems RRID:SCR_013673 Leica Thunder Computational Clearing (LVCC) Leica Microsystems Proprietary Imaris 10.1 Bitplane (Oxford Instruments) RRID:SCR_007370 LABKIT plugin (Fiji) Fiji/University of Heidelberg https://imagej.net/plugins/labkit 3D Object Manager (Fiji) ImageJ https://imagej.nih.gov/ij/plugins/track/ objects.html 3D MultiColoc (Fiji) ImageJ https://imagej.nih.gov/ij/plugins/multicolor- colocalization.html CropAndResize plugin CentrioleLab GitHub: https://github.com/CentrioleLab PickCentrioleDim plugin CentrioleLab GitHub: U-ExM-synapse-segmentation macro Cellular Immuno-Oncology Lausanne Zenodo: https://doi.org/10.5281/zenodo. ..

    Plasmid Preparation:

    Article Title: Unveiling the molecular architecture of T cells and immune synapses with cryo-expansion microscopy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies α-tubulin, rabbit ABCD antibodies Cat# AA345, clone scFv-F2C; RRID:AB_3106979 β-tubulin, mouse ABCD antibodies Cat# AA344, clone scFv-S11B; RRID:AB_3678982 β-actin, mouse Proteintech Cat# 60008-1-1, different lots; RRID:AB_2289225 30α-tubulin, rabbit Abcam Cat# ab18251, different lots; RRID:AB_2210057 TOMM20, rabbit Abcam Cat# ab186734, different lots; RRID:AB_2716623 LAMP1, sheep R and D systems Cat# AF4800, different lots; RRID:AB_1026176 C2CD3, rabbit Atlas antibodies Cat# HPA038552, different lots; RRID:AB_10669542 CEP164, rabbit Proteintech Cat# 22227-1-AP; RRID:AB_2651175 CEP170, rabbit Proteintech Cat# 27325-1-AP; RRID:AB_2880844 Granzyme B, goat R and D systems Cat# AF2906-SP; RRID:AB_622003 Granzyme B, mouse R and D systems Cat# MAB2906-SP, clone 351927; RRID:AB_2263752 Perforin, rabbit Proteintech Cat# 14580-1-AP, multiple lots; RRID:AB_10639524 CD8, mouse Proteintech Cat# 66868-1-Ig, clone 1G2B10; RRID:AB_2882205 CD4, rabbit Abcam Cat# ab133616; RRID:AB_2750883 CD2, rabbit Proteintech Cat# 23973-1-AP; RRID:AB_2879384 LCK, rabbit Atlas antibody Cat# HPA003494; RRID:AB_1852751 RAB7A, rabbit Proteintech Cat# 55469-1-AP; RRID:AB_11182831 RAB27A, rabbit Proteintech Cat# 66058-1-Ig; RRID:AB_11042596 VAMP7, rabbit Proteintech Cat# 22268-1-AP; RRID:AB_11182718 STX11, rabbit Proteintech Cat# 13301-1-AP; RRID:AB_2198214 SYTL2, rabbit Proteintech Cat# 12359-1-AP; RRID:AB_11182492 UNC13D, rabbit Proteintech Cat# 16905-1-AP; RRID:AB_2212713 TSP1, rabbit monoclonal Cell Signaling Cat# 37879, clone D7E5F; RRID:AB_2799123 Anti-mouse Alexa Fluor 568, goat Invitrogen Cat# A11029; RRID:AB_2534088 Anti-mouse Alexa Fluor 488, goat Invitrogen Cat# A11001; RRID:AB_2534069 Anti-Rabbit Alexa Fluor 488, goat Invitrogen Cat# A11008; RRID:AB_143165 Anti-Rabbit Alexa Fluor 568, goat Invitrogen Cat# A11036; RRID:AB_10563566 Anti-sheep Alexa Fluor 488, donkey Invitrogen Cat# A11015; RRID:AB_141362 Anti-rabbit Alexa Fluor Cy3, donkey Jackson Cat# 711-165-152; RRID:AB_2307443 Anti-Mouse Alexa Fluor 647, donkey Jackson Cat# 715-605-151; RRID:AB_2340863 Anti-Rabbit Alexa Fluor 647, donkey ThermoFisher Cat# A31573; RRID:AB_2536183 anti CD3 Alexa Fluor 700, flow cytometry BioLegend Cat# 300324; RRID:AB_493739 anti-CD4 − Pe-Cy7, flow cytometry BioLegend Cat# 317414; RRID:AB_571959 CD8, BV605, flow cytometry BioLegend Cat# 301040; RRID:AB_2563185 (Continued on next page) 22 Cell Reports 45, 117165, April 28, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER mouse TCRbeta- PE, flow cytometry BioLegend Cat#, 109208; RRID:AB_313431 Granzyme B- FITC, flow cytometry BD Cat#, 560211; RRID:AB_1645488 Perforin Alexa 647, flow cytometry BD Cat#, 563576; RRID:AB_2738287 Biological samples periphereal blood mononuclear cells from buffy coates of healthy human donors, completely anonymized and in Center of Interregional Blood Transfusion SRK Bern protocol P_503 human glioblastoma samples and melanoma brain metastass samples Geneva University Hospital protocol 2022-02109 protocol 2025-00256 Chemicals, peptides, and recombinant proteins Sodium acrylate home made no identifier available ICAM-1 Dustin lab, kind gift no identifier available mCLING-ATTO-Alexa Fluor 647N Source: Synaptic Systems Cat# 710 006AT1 NHS-Ester-Alexa Fluor 647 Source: Thermo Fisher Scientific Cat# A20006 NHS-Ester-Alexa Fluor 405 Source: Thermo Fisher Scientific Cat# A30000 Critical commercial assays CD4 isolation kit Miltenyi Biotech Cat# 130-096-533 CD8 isolation kit Miltenyi Biotech Cat# 130-096-495 Deposited data Jurlat, CTL SEM datasets OpenOrganelle jrc_jurkat-1, jrc_ctl-id8-1, jrc_ctl-id8-2 Experimental models: Cell lines U-2 OS American Type Culture Collection HTB-96 Jurkat, Clone E6-1 TIB-152 LGC STANDARDS GMBH TIB-152 B cell precursor leukemia BV173 German Cell Culture Collection (DSMZ) ACC 20 Recombinant DNA PEG plasmid Arber lab 101 available upon request PAM plasmid Arber lab 101 available upon request TCR plasmid Arber lab 101 available upon request TCR8 plasmid Arber lab 90 available upon request Software and algorithms Graph Pad Prism GraphPad Software, Inc. RRID:SCR_002798 Fiji NIH RRID:SCR_002285 R statistical environment R Foundation for Statistical Computing RRID:SCR_001905 LAS X software Leica Microsystems RRID:SCR_013673 Leica Thunder Computational Clearing (LVCC) Leica Microsystems Proprietary Imaris 10.1 Bitplane (Oxford Instruments) RRID:SCR_007370 LABKIT plugin (Fiji) Fiji/University of Heidelberg https://imagej.net/plugins/labkit 3D Object Manager (Fiji) ImageJ https://imagej.nih.gov/ij/plugins/track/ objects.html 3D MultiColoc (Fiji) ImageJ https://imagej.nih.gov/ij/plugins/multicolor- colocalization.html CropAndResize plugin CentrioleLab GitHub: https://github.com/CentrioleLab PickCentrioleDim plugin CentrioleLab GitHub: U-ExM-synapse-segmentation macro Cellular Immuno-Oncology Lausanne Zenodo: https://doi.org/10.5281/zenodo. ..

    Software:

    Article Title: Unveiling the molecular architecture of T cells and immune synapses with cryo-expansion microscopy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies α-tubulin, rabbit ABCD antibodies Cat# AA345, clone scFv-F2C; RRID:AB_3106979 β-tubulin, mouse ABCD antibodies Cat# AA344, clone scFv-S11B; RRID:AB_3678982 β-actin, mouse Proteintech Cat# 60008-1-1, different lots; RRID:AB_2289225 30α-tubulin, rabbit Abcam Cat# ab18251, different lots; RRID:AB_2210057 TOMM20, rabbit Abcam Cat# ab186734, different lots; RRID:AB_2716623 LAMP1, sheep R and D systems Cat# AF4800, different lots; RRID:AB_1026176 C2CD3, rabbit Atlas antibodies Cat# HPA038552, different lots; RRID:AB_10669542 CEP164, rabbit Proteintech Cat# 22227-1-AP; RRID:AB_2651175 CEP170, rabbit Proteintech Cat# 27325-1-AP; RRID:AB_2880844 Granzyme B, goat R and D systems Cat# AF2906-SP; RRID:AB_622003 Granzyme B, mouse R and D systems Cat# MAB2906-SP, clone 351927; RRID:AB_2263752 Perforin, rabbit Proteintech Cat# 14580-1-AP, multiple lots; RRID:AB_10639524 CD8, mouse Proteintech Cat# 66868-1-Ig, clone 1G2B10; RRID:AB_2882205 CD4, rabbit Abcam Cat# ab133616; RRID:AB_2750883 CD2, rabbit Proteintech Cat# 23973-1-AP; RRID:AB_2879384 LCK, rabbit Atlas antibody Cat# HPA003494; RRID:AB_1852751 RAB7A, rabbit Proteintech Cat# 55469-1-AP; RRID:AB_11182831 RAB27A, rabbit Proteintech Cat# 66058-1-Ig; RRID:AB_11042596 VAMP7, rabbit Proteintech Cat# 22268-1-AP; RRID:AB_11182718 STX11, rabbit Proteintech Cat# 13301-1-AP; RRID:AB_2198214 SYTL2, rabbit Proteintech Cat# 12359-1-AP; RRID:AB_11182492 UNC13D, rabbit Proteintech Cat# 16905-1-AP; RRID:AB_2212713 TSP1, rabbit monoclonal Cell Signaling Cat# 37879, clone D7E5F; RRID:AB_2799123 Anti-mouse Alexa Fluor 568, goat Invitrogen Cat# A11029; RRID:AB_2534088 Anti-mouse Alexa Fluor 488, goat Invitrogen Cat# A11001; RRID:AB_2534069 Anti-Rabbit Alexa Fluor 488, goat Invitrogen Cat# A11008; RRID:AB_143165 Anti-Rabbit Alexa Fluor 568, goat Invitrogen Cat# A11036; RRID:AB_10563566 Anti-sheep Alexa Fluor 488, donkey Invitrogen Cat# A11015; RRID:AB_141362 Anti-rabbit Alexa Fluor Cy3, donkey Jackson Cat# 711-165-152; RRID:AB_2307443 Anti-Mouse Alexa Fluor 647, donkey Jackson Cat# 715-605-151; RRID:AB_2340863 Anti-Rabbit Alexa Fluor 647, donkey ThermoFisher Cat# A31573; RRID:AB_2536183 anti CD3 Alexa Fluor 700, flow cytometry BioLegend Cat# 300324; RRID:AB_493739 anti-CD4 − Pe-Cy7, flow cytometry BioLegend Cat# 317414; RRID:AB_571959 CD8, BV605, flow cytometry BioLegend Cat# 301040; RRID:AB_2563185 (Continued on next page) 22 Cell Reports 45, 117165, April 28, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER mouse TCRbeta- PE, flow cytometry BioLegend Cat#, 109208; RRID:AB_313431 Granzyme B- FITC, flow cytometry BD Cat#, 560211; RRID:AB_1645488 Perforin Alexa 647, flow cytometry BD Cat#, 563576; RRID:AB_2738287 Biological samples periphereal blood mononuclear cells from buffy coates of healthy human donors, completely anonymized and in Center of Interregional Blood Transfusion SRK Bern protocol P_503 human glioblastoma samples and melanoma brain metastass samples Geneva University Hospital protocol 2022-02109 protocol 2025-00256 Chemicals, peptides, and recombinant proteins Sodium acrylate home made no identifier available ICAM-1 Dustin lab, kind gift no identifier available mCLING-ATTO-Alexa Fluor 647N Source: Synaptic Systems Cat# 710 006AT1 NHS-Ester-Alexa Fluor 647 Source: Thermo Fisher Scientific Cat# A20006 NHS-Ester-Alexa Fluor 405 Source: Thermo Fisher Scientific Cat# A30000 Critical commercial assays CD4 isolation kit Miltenyi Biotech Cat# 130-096-533 CD8 isolation kit Miltenyi Biotech Cat# 130-096-495 Deposited data Jurlat, CTL SEM datasets OpenOrganelle jrc_jurkat-1, jrc_ctl-id8-1, jrc_ctl-id8-2 Experimental models: Cell lines U-2 OS American Type Culture Collection HTB-96 Jurkat, Clone E6-1 TIB-152 LGC STANDARDS GMBH TIB-152 B cell precursor leukemia BV173 German Cell Culture Collection (DSMZ) ACC 20 Recombinant DNA PEG plasmid Arber lab 101 available upon request PAM plasmid Arber lab 101 available upon request TCR plasmid Arber lab 101 available upon request TCR8 plasmid Arber lab 90 available upon request Software and algorithms Graph Pad Prism GraphPad Software, Inc. RRID:SCR_002798 Fiji NIH RRID:SCR_002285 R statistical environment R Foundation for Statistical Computing RRID:SCR_001905 LAS X software Leica Microsystems RRID:SCR_013673 Leica Thunder Computational Clearing (LVCC) Leica Microsystems Proprietary Imaris 10.1 Bitplane (Oxford Instruments) RRID:SCR_007370 LABKIT plugin (Fiji) Fiji/University of Heidelberg https://imagej.net/plugins/labkit 3D Object Manager (Fiji) ImageJ https://imagej.nih.gov/ij/plugins/track/ objects.html 3D MultiColoc (Fiji) ImageJ https://imagej.nih.gov/ij/plugins/multicolor- colocalization.html CropAndResize plugin CentrioleLab GitHub: https://github.com/CentrioleLab PickCentrioleDim plugin CentrioleLab GitHub: U-ExM-synapse-segmentation macro Cellular Immuno-Oncology Lausanne Zenodo: https://doi.org/10.5281/zenodo. ..



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